Enforced Expression of GATA-3 Severely Reduces Human Thymic Cellularity

Enforced expression of GATA-3 severely reduces human thymic cellularity.

Following bone marrow transplantation, patients often suffer from immune incompetence by reduced or late T cell development. Moreover, adult bone marrow stem cells have a lower capacity to generate T cells compared with fetal liver- and umbilical cord blood-derived progenitors. Therefore, enhancing thymic-dependent T cell generation might hold great therapeutic potential. GATA-3 is a transcript...

Lymphoma in Transgenic Mice Single-Positive Cells and Induces Thymic CD8 Cell Development Inhibits Maturation of Enforced Expression of GATA-3 During T

Enforced expression of GATA-3 during T cell development inhibits maturation of CD8 single-positive cells and induces thymic lymphoma in transgenic mice.

The zinc finger transcription factor GATA-3 is of critical importance for early T cell development and commitment of Th2 cells. To study the role of GATA-3 in early T cell development, we analyzed and modified GATA-3 expression in vivo. In mice carrying a targeted insertion of a lacZ reporter on one allele, we found that GATA-3 transcription in CD4(+)CD8(+) double-positive thymocytes correlated...

Embryonic expression of the human GATA-3 gene

The spatial and temporal analysis of GATA-3 expression pattern in the human embryo revealed its expression in new anatomical sites. These include the endoderm of the primitive foregut, pharynx and allantois, the branchial arches and the mesenchymal cells surrounding the stomach and dorsal aorta. On the other hand, human (h) GATA-3 expression in the central nervous system, somites and embryonic ...

Wnt4 regulates thymic cellularity through the expansion of thymic epithelial cells and early thymic progenitors.

Thymus atrophy is the most common immunopathology in humans, and its occurrence is hastened by several factors that coalesce in patients receiving chemotherapy and most of all in recipients of hematopoietic cell transplantation. We have shown previously that posthematopoietic cell transplantation thymic function was improved by retroviral overexpression of Wnt4 in donor hematopoietic cells. Her...